KAP1 [p Ser824] Antibody (BL-246-7B5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76398
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BL-246-7B5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155)
Modification
p Ser824
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KAP1 [p Ser824] Antibody (BL-246-7B5)
Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] -
Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Applications for KAP1 [p Ser824] Antibody (BL-246-7B5)
Application
Recommended Usage
Flow Cytometry
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
20 ul/mg lysate
Simple Western
1:10 - 1:250
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Gene Symbol
TRIM28
Additional KAP1 Products
Product Documents for KAP1 [p Ser824] Antibody (BL-246-7B5)
Product Specific Notices for KAP1 [p Ser824] Antibody (BL-246-7B5)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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