KAT1 Antibody (7U2O3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16412

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16412-100ul
NBP3-16412-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7U2O3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KAT1 Antibodies »

Product Summary for KAT1 Antibody (7U2O3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human KAT1 (NP_003633.2). HYFLVFEKYNKDGATLFATVGYMTVYNYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSKSYVKLRDFVLVKLCQDLPCFSREKLMQGFNEDMVIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAT1 Antibody (7U2O3)

Western Blot: KAT1 Antibody (7U2O3) [NBP3-16412]

Western Blot: KAT1 Antibody (7U2O3) [NBP3-16412]

Western Blot: KAT1 Antibody (7U2O3) [NBP3-16412] - Western blot analysis of extracts of various cell lines, using KAT1 Rabbit mAb (NBP3-16412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: KAT1 Antibody (7U2O3) [NBP3-16412]

Immunocytochemistry/ Immunofluorescence: KAT1 Antibody (7U2O3) [NBP3-16412]

Immunocytochemistry/Immunofluorescence: KAT1 Antibody (7U2O3) [NBP3-16412] - Immunofluorescence analysis of NIH-3T3 cells using KAT1 Rabbit mAb (NBP3-16412) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: KAT1 Antibody (7U2O3) [NBP3-16412]

Immunohistochemistry-Paraffin: KAT1 Antibody (7U2O3) [NBP3-16412]

Immunohistochemistry-Paraffin: KAT1 Antibody (7U2O3) [NBP3-16412] - Immunohistochemistry of paraffin-embedded mouse testis using KAT1 Rabbit mAb (NBP3-16412) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for KAT1 Antibody (7U2O3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KAT1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Alternate Names

EC 2.3.1.48, histone acetyltransferase 1, histone acetyltransferase type B catalytic subunit, KAT1

Gene Symbol

HAT1

Additional KAT1 Products

Product Documents for KAT1 Antibody (7U2O3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KAT1 Antibody (7U2O3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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