KAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98803

Novus Biologicals, part of Bio-Techne
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NBP2-98803-100ul
NBP2-98803-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KAT1 Antibodies »

Product Summary for KAT1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human KAT1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for KAT1 Antibody

Western Blot: KAT1 Antibody [NBP2-98803]

Western Blot: KAT1 Antibody [NBP2-98803]

Western Blot: KAT1 Antibody [NBP2-98803] - Anti-KAT1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: U87MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa
Immunocytochemistry/ Immunofluorescence: KAT1 Antibody [NBP2-98803]

Immunocytochemistry/ Immunofluorescence: KAT1 Antibody [NBP2-98803]

Immunocytochemistry/Immunofluorescence: KAT1 Antibody [NBP2-98803] - Immunofluorescence staining of KAT1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human KAT1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Applications for KAT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAT1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Alternate Names

EC 2.3.1.48, histone acetyltransferase 1, histone acetyltransferase type B catalytic subunit, KAT1

Gene Symbol

HAT1

Additional KAT1 Products

Product Documents for KAT1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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