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KAT4/TBP Associated Factor 1 Antibody (1G9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006872-M02

Novus Biologicals, part of Bio-Techne
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H00006872-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TAF1 (NP_004597, 1784 a.a. ~ 1893 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Specificity

TAF1 (1G9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KAT4/TBP Associated Factor 1 Antibody (1G9)

Western Blot: KAT4/TBP Associated Factor 1 Antibody (1G9) [H00006872-M02]

Western Blot: KAT4/TBP Associated Factor 1 Antibody (1G9) [H00006872-M02]

Western Blot: KAT4/TBP Associated Factor 1 Antibody (1G9) [H00006872-M02] - TAF1 monoclonal antibody (M02), clone 1G9 Analysis of TAF1 expression in Hela S3 NE.

Applications for KAT4/TBP Associated Factor 1 Antibody (1G9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KAT4/TBP Associated Factor 1

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.

Long Name

Transcription initiation factor TFIID subunit 1

Alternate Names

BA2R, CCG1, CCGS, p250, TAF(II)250, TAF1, TAF2A, TAFII-250, TAFII250

Entrez Gene IDs

6872 (Human)

Gene Symbol

TAF1

OMIM

313650 (Human)

Additional KAT4/TBP Associated Factor 1 Products

Product Documents for KAT4/TBP Associated Factor 1 Antibody (1G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KAT4/TBP Associated Factor 1 Antibody (1G9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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