katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71578

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all katanin-p80 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human KATNB1(NP_005877) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

Western Blot: katanin-p80 Antibody (OTI5C4)Azide and BSA Free [NBP2-71578]

Western Blot: katanin-p80 Antibody (OTI5C4)Azide and BSA Free [NBP2-71578]

Western Blot: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY katanin-p80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-katanin-p8
Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578]

Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578]

Immunocytochemistry/Immunofluorescence: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY katanin-p80.
Immunohistochemistry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578]

Immunohistochemistry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578]

Immunohistochemistry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - Staining of paraffin-embedded Human colon tissue using anti-katanin-p80 mouse monoclonal antibody.
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Applications for katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: katanin-p80

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help toorganize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemblemicrotubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family ofATPases. (provided by RefSeq)

Alternate Names

KAT, katanin (80 kDa), katanin p80 (WD repeat containing) subunit B 1, katanin p80 (WD40-containing) subunit B 1, Katanin p80 subunit B1, katanin p80 WD40-containing subunit B1, p80 katanin

Gene Symbol

KATNB1

Additional katanin-p80 Products

Product Documents for katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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