katanin-p80 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87413

Novus Biologicals, part of Bio-Techne
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NBP1-87413
NBP1-87413-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all katanin-p80 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPAMDVQFPVPNLEVLPRPPVVASTPAPKAEPAIIPATRNEPIGLKASDFLPAVKIPQQAELVDEDAMSQIRKGHDTMCVVLTSRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for katanin-p80 Antibody

Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody [NBP1-87413]

Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody [NBP1-87413]

Immunocytochemistry/Immunofluorescence: katanin-p80 Antibody [NBP1-87413] - Katanin p60 and p80 expression in the mouse testis. Immunohistochemical localization of katanin p60 (right) and p80 (left) in the mouse seminiferous epithelium. Katanin subunits?=?red, alpha-tubulin?=?green, nuclei?=?blue. Both katanin subunits were prominently localized to elongating spermatids (arrowheads). Katanin p60 was also visible in Sertoli cell nuclei (SC). The inset in each micrograph shows a control for the secondary antibody (primary antibody substituted with buffer). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1002698), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: katanin-p80 Antibody [NBP1-87413]

Immunohistochemistry-Paraffin: katanin-p80 Antibody [NBP1-87413]

Immunohistochemistry-Paraffin: katanin-p80 Antibody [NBP1-87413] - Staining in human testis and pancreas tissues using anti-KATNB1 antibody. Corresponding KATNB1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody [NBP1-87413]

Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody [NBP1-87413]

Immunocytochemistry/Immunofluorescence: katanin-p80 Antibody [NBP1-87413] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and microtubules.
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Applications for katanin-p80 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: katanin-p80

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help toorganize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemblemicrotubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family ofATPases. (provided by RefSeq)

Alternate Names

KAT, katanin (80 kDa), katanin p80 (WD repeat containing) subunit B 1, katanin p80 (WD40-containing) subunit B 1, Katanin p80 subunit B1, katanin p80 WD40-containing subunit B1, p80 katanin

Entrez Gene IDs

10300 (Human)

Gene Symbol

KATNB1

UniProt

Q9BVA0 (Human)

Additional katanin-p80 Products

Product Documents for katanin-p80 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for katanin-p80 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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