KBTBD10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80786

Novus Biologicals, part of Bio-Techne
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NBP1-80786
NBP1-80786-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Mouse, Bovine

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KBTBD10 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDKSLPCHRLILSACSPYFREYFLSEIDEAKKKEVVLDNVDPAILDLIIKYLYSASIDLNDGNVQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KBTBD10 Antibody

Western Blot: KBTBD10 Antibody [NBP1-80786]

Western Blot: KBTBD10 Antibody [NBP1-80786]

Western Blot: KBTBD10 Antibody [NBP1-80786] - Analysis in control (vector only transfected HEK293T lysate) and KLHL41 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: KBTBD10 Antibody [NBP1-80786]

Immunocytochemistry/ Immunofluorescence: KBTBD10 Antibody [NBP1-80786]

Immunocytochemistry/Immunofluorescence: KBTBD10 Antibody [NBP1-80786] - Staining of human cell line A-431 shows positivity in nucleus, plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: KBTBD10 Antibody [NBP1-80786]

Immunohistochemistry-Paraffin: KBTBD10 Antibody [NBP1-80786]

Immunohistochemistry-Paraffin: KBTBD10 Antibody [NBP1-80786] - Staining of human colon.
View 5 more images

Applications for KBTBD10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KBTBD10

KBTBD10 is required for pseudopod elongation in transformed cells. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins

Alternate Names

FLJ60989, kelch repeat and BTB (POZ) domain containing 10, kelch repeat and BTB domain-containing protein 10, Kelch-related protein 1, Kel-like protein 23, KRP1, Sarcosin, SARCOSINsarcomeric muscle protein

Entrez Gene IDs

10324 (Human)

Gene Symbol

KBTBD10

Additional KBTBD10 Products

Product Documents for KBTBD10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KBTBD10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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