KCC2/SLC12A5 Antibody (S1-12)

Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337]

Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.

Immunocytochemistry/ Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337]

Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2/SLC12A5 Antibody (D) Composite.

Immunocytochemistry/ Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337]

Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.