KCMF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84283

Novus Biologicals, part of Bio-Techne
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NBP1-84283
NBP1-84283-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KCMF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSETERQSMESERADRSLFVQELLLSTLVREESSSSDEDDRGEMADFGAMGCVDIMPLDVALENLNLKESNKGNEPPPPPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KCMF1 Antibody

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84283]

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84283]

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84283] - Staining of human colon, kidney, liver and testis using Anti-KCMF1 antibody NBP1-84283 (A) shows similar protein distribution across tissues to independent antibody NBP1-84284 (B).
Western Blot: KCMF1 Antibody [NBP1-84283]

Western Blot: KCMF1 Antibody [NBP1-84283]

Western Blot: KCMF1 Antibody [NBP1-84283] - Analysis in control (vector only transfected HEK293T lysate) and KCMF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: KCMF1 Antibody [NBP1-84283]

Immunocytochemistry/ Immunofluorescence: KCMF1 Antibody [NBP1-84283]

Immunocytochemistry/Immunofluorescence: KCMF1 Antibody [NBP1-84283] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
View 4 more images

Applications for KCMF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KCMF1

KCMF1 has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination

Alternate Names

DEBT91, DKFZP434L1021, EC 6.3.2, EC 6.3.2.-, FGF-induced in gastric cancer, FGF-induced ubiquitin-protein ligase in gastric cancers, FIGC, PCMFdifferentially expressed in branching tubulogenesis 91, Potassium channel modulatory factor, potassium channel modulatory factor 1, zinc finger, ZZ domain containing 1, ZZ-type zinc finger-containing protein 1, ZZZ1E3 ubiquitin-protein ligase KCMF1

Gene Symbol

KCMF1

Additional KCMF1 Products

Product Documents for KCMF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KCMF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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