KCNJ10 Antibody (1C11)
Novus Biologicals, part of Bio-Techne | Catalog # H00003766-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
ELISA, Immunohistochemistry, Immunoprecipitation, Sandwich ELISA, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1C11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KCNJ10 (NP_002232, 276 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30542265). Rat reactivity reported in scientific literature (PMID: 19009624).
Specificity
KCNJ10 - potassium inwardly-rectifying channel, subfamily J, member 10
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for KCNJ10 Antibody (1C11)
![Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01]](https://resources.bio-techne.com/images/products/KCNJ10-Antibody-1C11-Immunohistochemistry-H00003766-M01-img0003.jpg "Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01]")
Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01]
Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] - Cx43, Cx30, and KCNJ10 (Kir4.1) (H00003766-M01) immunoreactivity. Immunoreactivity for Cx43 was maximal at P12 and decayed with age, whereas Cx30 levels increased at later developmental stages. Kir4.1 exhibited a moderately elevated level during the second postnatal week. The last row depicts negative controls at P20, in which the primary antibodies for Cx43 and Cx30 were omitted.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542265/) licensed under a CC-BY license.
Sandwich ELISA: KCNJ10 Antibody (1C11) [H00003766-M01] - Detection limit for recombinant GST tagged KCNJ10 is approximately 0.03ng/ml as a capture antibody.
Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] -
Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] - Cx43, Cx30, & Kir4.1 immunoreactivity. (A) Schematic drawing of an auditory brainstem slice containing the central part of the inferior colliculus (ICC; A1). The tonotopic organization is indicated by dotted lines (A2). The dotted box denotes the area in which Cx expression was analyzed. (B) Immunoreactivity for Cx43 was maximal at P12 & decayed with age, whereas Cx30 levels increased at later developmental stages. Kir4.1 exhibited a moderately elevated level during the second postnatal week. The last row depicts negative controls at P20, in which the primary antibodies for Cx43 & Cx30 were omitted. (C) Developmental profile of Cx43 (C1), Cx30 (C2), & Kir4.1 expression (C3). The dotted gray lines in (C1,2) denote developmental background fluorescence signals at P7–30 obtained after omitting primary antibodies. n represents the number of analyzed slices & is provided within the diagrams; shown are mean values ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30542265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for KCNJ10 Antibody (1C11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. IHC usage reported in scientific literature (PMID: 27458984).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: KCNJ10
Long Name
ATP-sensitive inward rectifier potassium channel 10
Alternate Names
ATP-dependent inwardly rectifying potassium channel Kir4.1, ATP-sensitive inward rectifier potassium channel 10, BIRK-10, glial ATP-dependent inwardly rectifying potassium channel KIR4.1, Inward rectifier K(+) channel Kir1.2, inward rectifier K+ channel KIR1.2, KCNJ13-PEN, KIR1.2, Kir4.1, Potassium channel, inwardly rectifying subfamily J member 10, potassium inwardly-rectifying channel, subfamily J, member 10, SESAME
Entrez Gene IDs
3766 (Human)
Gene Symbol
KCNJ10
OMIM
602208 (Human)
UniProt
Additional KCNJ10 Products
Product Documents for KCNJ10 Antibody (1C11)
Product Specific Notices for KCNJ10 Antibody (1C11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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