KCNMB2 Antibody (S53-32)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12917

Novus Biologicals, part of Bio-Techne
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NBP2-12917-0.025mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S53-32

Concentration

1 mg/ml

View all KCNMB2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2

Localization

Membrane

Specificity

Detects approx 27kDa. No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KCNMB2 Antibody (S53-32)

Western Blot: KCNMB2 Antibody (S53-32) [NBP2-12917]

Western Blot: KCNMB2 Antibody (S53-32) [NBP2-12917]

Western Blot: KCNMB2 Antibody (S53-32) [NBP2-12917] - analysis of Human Cell line lysates showing detection of BK Beta2 Potassium Channel protein using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: KCNMB2 Antibody (S53-32) [NBP2-12917]

Immunocytochemistry/ Immunofluorescence: KCNMB2 Antibody (S53-32) [NBP2-12917]

Immunocytochemistry/Immunofluorescence: KCNMB2 Antibody (S53-32) [NBP2-12917] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dottiness and dull.
Immunohistochemistry-Paraffin: KCNMB2 Antibody (S53-32) [NBP2-12917]

Immunohistochemistry-Paraffin: KCNMB2 Antibody (S53-32) [NBP2-12917]

Immunohistochemistry-Paraffin: KCNMB2 Antibody (S53-32) [NBP2-12917] - . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower epidermal cells positive, but quite dull.

Applications for KCNMB2 Antibody (S53-32)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000
Application Notes
1 ug/ml of BK Beta 2 Antibody was sufficient for detection of BK Channel beta2 in 10 ug of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KCNMB2

1 ug/mL was sufficient for detection of BK Channel beta2 in 10ug of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

BK channel subunit beta-2, BKbeta2, calcium-activated potassium channel subunit beta-2, Calcium-activated potassium channel, subfamily M subunit beta-2, Charybdotoxin receptor subunit beta-2, Hbeta2, hbeta3, K(VCA)beta-2, large conductance calcium-activated potassium channel beta 2 subunit, large-conductance Ca2+-activated K+ channel beta2 subunit, Maxi K channel subunit beta-2, MaxiK channel beta 2 subunit, MGC22431, potassium large conductance calcium-activated channel, subfamily M, beta member2, Slo-beta-2

Gene Symbol

KCNMB2

Additional KCNMB2 Products

Product Documents for KCNMB2 Antibody (S53-32)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KCNMB2 Antibody (S53-32)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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