KCNT2 Antibody (S11-33)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47606

Novus Biologicals, part of Bio-Techne
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NBP1-47606-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S11-33

Concentration

1 mg/ml

View all KCNT2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 564-624 of mouse Slo2.1 (Slick)

Localization

Cell Membrane

Specificity

Detects approx 140kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KCNT2 Antibody (S11-33)

Western Blot: KCNT2 Antibody (S11-33) [NBP1-47606]

Western Blot: KCNT2 Antibody (S11-33) [NBP1-47606]

Western Blot: KCNT2 Antibody (S11-33) [NBP1-47606] - analysis of Rat brain membrane lysate showing detection of Slo2.1 Potassium Channel protein using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: KCNT2 Antibody (S11-33) [NBP1-47606]

Immunocytochemistry/ Immunofluorescence: KCNT2 Antibody (S11-33) [NBP1-47606]

Immunocytochemistry/Immunofluorescence: KCNT2 Antibody (S11-33) [NBP1-47606] - analysis using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive (nuclei and cytoplasm).
Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606]

Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606]

Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.
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Applications for KCNT2 Antibody (S11-33)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

0.1-1.0 ug/ml

Western Blot

1-10 ug/ml
Application Notes
1 ug/ml of Slo2.1 Antibody was sufficient for detection of Slo2.1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KCNT2

FUCNTION: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. Activated by high intracellular sodium and chloride levels. Channel activity is inhibited by ATP and by inhalation anesthetics, such as isoflurane. Inhibited upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1.; Subcellular location: Cell membrane; Multi-pass membrane protein

Alternate Names

KCa4.2, MGC119610, MGC119611, MGC119612, potassium channel subfamily T member 2, potassium channel, subfamily T, member 2, Sequence like an intermediate conductance potassium channel subunit, SLICKMGC119613, SLO2.1, sodium activated potassium channel, sodium and chloride activated ATP sensitive potassium channel, sodium- and chloride-activated ATP-sensitive potassium channel, Sodium and chloride-activated ATP-sensitive potassium channel Slo2.1, sodium-and chloride-activated ATP-sensitive potassium channel (SLICK)

Entrez Gene IDs

343450 (Human)

Gene Symbol

KCNT2

UniProt

Q6UVM3 (Human)

Additional KCNT2 Products

Product Documents for KCNT2 Antibody (S11-33)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KCNT2 Antibody (S11-33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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