KDELR1 Antibody (KR-10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12873
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Hamster, Mammal, Monkey, Rabbit, Sheep, Xenopus
Cited:
Mammal
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # KR-10
Concentration
1 mg/ml
Product Specifications
Immunogen
A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
Reactivity Notes
Use in Mammal reported in scientific literature (PMID:31653700).
Localization
Endoplasmic Reticulum
Specificity
Detects approx 25kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for KDELR1 Antibody (KR-10)
Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873]
Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873] - analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/ Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873]
Immunocytochemistry/Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDELR1 Monoclonal Antibody, Clone KR-10 (NBP2-12873). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDELR1 Monoclonal Antibody (NBP2-12873) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.Applications for KDELR1 Antibody (KR-10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000
Application Notes
1 ug/ml was sufficient for detection of KDEL receptor in 20 ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Reviewed Applications
Read 1 review rated 4 using NBP2-12873 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KDELR1
Alternate Names
ER lumen protein retaining receptor 1, ERD2, ERD2.1KDEL receptor 1, HDEL, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, PM23, Putative MAPK-activating protein PM23
Gene Symbol
KDELR1
UniProt
Additional KDELR1 Products
Product Documents for KDELR1 Antibody (KR-10)
Product Specific Notices for KDELR1 Antibody (KR-10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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