KDM2A/FBXL11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74602
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Chemotaxis, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for KDM2A/FBXL11 Antibody
Western Blot: KDM2A/FBXL11 Antibody [NB100-74602]
Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of Human JHDM1A by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug) and 293T (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody NB100-74602 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- JHDM1A used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Analysis of human lung tumor tissue using anti-KDM2A antibody. Image submitted by a verified customer review.Applications for KDM2A/FBXL11 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30132864). KDM2A/FBXL11 antibody validated for IHC-P from a verified customer review.
Reviewed Applications
Read 2 reviews rated 5 using NB100-74602 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: KDM2A/FBXL11
Long Name
Lysine-specific demethylase 2A
Alternate Names
CXXC8, EC 1.14.11.27, F-box protein FBL7, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004
Entrez Gene IDs
22992 (Human)
Gene Symbol
KDM2A
UniProt
Additional KDM2A/FBXL11 Products
Product Documents for KDM2A/FBXL11 Antibody
Product Specific Notices for KDM2A/FBXL11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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