KDM2B Antibody (5G1)

Novus Biologicals, part of Bio-Techne | Catalog # H00084678-M09

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5G1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KDM2B Antibodies »

Product Specifications

Immunogen

FBXL10 (NP_115979, 457 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEH

Specificity

FBXL10 - F-box and leucine-rich repeat protein 10

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for KDM2B Antibody (5G1)

Western Blot: KDM2B Antibody (5G1) [H00084678-M09]

Western Blot: KDM2B Antibody (5G1) [H00084678-M09]

Western Blot: KDM2B Antibody (5G1) [H00084678-M09] - Western Blot analysis of FBXL10 expression in Hela NE ( Cat # L013V3 ).
Immunocytochemistry/ Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09]

Immunocytochemistry/ Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09]

Immunocytochemistry/Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09] - Analysis of monoclonal antibody to FBXL10 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: KDM2B Antibody (5G1) [H00084678-M09] - Detection limit for recombinant GST tagged FBXL10 is approximately 0.1ng/ml as a capture antibody.

Applications for KDM2B Antibody (5G1)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KDM2B

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Long Name

Lysine-specific demethylase 2B

Alternate Names

CXXC2, F-box protein FBL10, FBL10, FBXL10, JHDM1B, NDY1, PCCX2, Protein JEMMA

Entrez Gene IDs

84678 (Human)

Gene Symbol

KDM2B

OMIM

609078 (Human)

UniProt

NP_115979 (Human)

Additional KDM2B Products

Product Documents for KDM2B Antibody (5G1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KDM2B Antibody (5G1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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