KF1 Antibody (3E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00007844-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF103 (NP_005658, 501 a.a. ~ 599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYN
Specificity
RNF103 - ring finger protein 103
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for KF1 Antibody (3E7)
![Western Blot: KF1 Antibody (3E7) [H00007844-M01]](https://resources.bio-techne.com/images/products/KF1-Antibody-3E7-Western-Blot-H00007844-M01-img0003.jpg "Western Blot: KF1 Antibody (3E7) [H00007844-M01]")
Western Blot: KF1 Antibody (3E7) [H00007844-M01]
Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 over-expressed 293 cell line, cotransfected with RNF103 Validated Chimera RNAi ( Cat # H00007844-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF103 monoclonal antibody (M01), clone 3E7 (Cat # H00007844-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: KF1 Antibody (3E7) [H00007844-M01]](https://resources.bio-techne.com/images/products/KF1-Antibody-3E7-Western-Blot-H00007844-M01-img0004.jpg "Western Blot: KF1 Antibody (3E7) [H00007844-M01]")
Western Blot: KF1 Antibody (3E7) [H00007844-M01]
Western Blot: KF1 Antibody (3E7) [H00007844-M01] - Analysis of RNF103 expression in transfected 293T cell line by RNF103 monoclonal antibody (M01), clone 3E7.Lane 1: RNF103 transfected lysate(79.405 KDa).Lane 2: Non-transfected lysate.Applications for KF1 Antibody (3E7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: KF1
Alternate Names
E3 ubiquitin-protein ligase RNF103, EC 6.3.2.-, hKF-1, KF-1, KF1ZFP103ZFP-103, MGC102815, ring finger protein 103MGC41857, Zfp-103, Zinc finger protein 103 homolog, zinc finger protein 103 homolog (mouse), zinc finger protein expressed in cerebellum
Entrez Gene IDs
7844 (Human)
Gene Symbol
RNF103
OMIM
602507 (Human)
UniProt
Additional KF1 Products
Product Documents for KF1 Antibody (3E7)
Product Specific Notices for KF1 Antibody (3E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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