Kif2a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-180

Novus Biologicals, part of Bio-Techne
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NB500-180
NB500-180SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Mammal

Cited:

Human, Mouse, Porcine, Bovine, Insect - Drosophila melanogaster, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Kif2a Antibodies »

Product Specifications

Immunogen

A recombinant segment of the N-terminal domain of human Kif2a. [UniProt# O00139]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kif2a Antibody

Knockout Validated: Kif2a Antibody [NB500-180]

Knockout Validated: Kif2a Antibody [NB500-180]

Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Kif2a Antibody [NB500-180]

Western Blot: Kif2a Antibody [NB500-180]

Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.
Immunocytochemistry/ Immunofluorescence: Kif2a Antibody [NB500-180]

Immunocytochemistry/ Immunofluorescence: Kif2a Antibody [NB500-180]

Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Applications for Kif2a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunoprecipitation

1:10-1:500

Simple Western

1:1000

Western Blot

1:1000
Application Notes
This Kif2a antibody is useful for Western blot, Immunoprecipitation, and Immunocytochemistry/Immunofluorescence. A band at approx. 110 kDa can be detected by Western blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Kif2a

Kif2a (Kinesin-like protein KIF2A or Kinesin-2) belongs to Kinesin-13 family that includes Kif2a, Kif2b and Kif2c members which are classified as microtubule depolymerases with potential of depolymerizing microtubules at their ends. Among these proteins, Kif2a specifically localizes to centrosomes during mitotic cell division and is essential to normal congress of chromosomes at the metaphase plate, normal spindle dynamics/turnover and for the formation of bipolar mitotic spindles. Kif2a gets localized to the spindle microtubules and spindle poles from prophase to metaphase. Kif2a-PSRC1 interactions leads to its recruitment to mitotic spindles, PLK1 kiase activity is required for Kif2a targeting to spindle microtubules and spindle poles. siRNA knockdown of Kif2a in human cancer cells or Kif2a blocking with antibody addition to Xenopus eggs leads to monopolar spindles formation and cell cycle block suggesting that Kif2a is essential for bipolar spindle assembly and normal progression of mitosis. Kif2a has been shown to be essential for proper neuronal outgrowth and its depletion caused neuronal migratory delay by changing microtubule dynamics around the cell body suggesting that Kif2a is implicated in non-mitotic cells also. Moreover, Kif2a has been established as a plus end-directed microtubule-dependent motor essential for normal brain development and that it regulates microtubule dynamics during axonal growth.

Alternate Names

HK2, KIF2kinesin-2, kinesin heavy chain member 2, kinesin heavy chain member 2A, Kinesin, heavy chain, 2, Kinesin-2, kinesin-like protein KIF2A, KNS2

Entrez Gene IDs

3796 (Human); 16563 (Mouse); 84391 (Rat)

Gene Symbol

KIF2A

UniProt

O00139 (Human)

Additional Kif2a Products

Product Documents for Kif2a Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Kif2a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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