Kif2a Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83253
![Western Blot: Kif2a Antibody [NBP1-83253]](https://resources.bio-techne.com/images/products/Kif2a-Antibody-Western-Blot-NBP1-83253-img0004.jpg "Western Blot: Kif2a Antibody [NBP1-83253]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat
Predicted:
Mouse (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DNESVTVEWIENGDTKGKEIDLESIFSLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Kif2a Antibody
![Knockout Validated: Kif2a Antibody [NBP1-83253]](https://resources.bio-techne.com/images/products/Kif2a-Antibody-Knockout-Validated-NBP1-83253-img0006.jpg "Knockout Validated: Kif2a Antibody [NBP1-83253]")
Knockout Validated: Kif2a Antibody [NBP1-83253]
Knockout Validated: Kif2a Antibody [NBP1-83253] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NBP1-83253) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.![Western Blot: Kif2a Antibody [NBP1-83253]](https://resources.bio-techne.com/images/products/Kif2a-Antibody-Western-Blot-NBP1-83253-img0005.jpg "Western Blot: Kif2a Antibody [NBP1-83253]")
Western Blot: Kif2a Antibody [NBP1-83253]
Western Blot: Kif2a Antibody [NBP1-83253] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253]](https://resources.bio-techne.com/images/products/Kif2a-Antibody-Immunohistochemistry-Paraffin-NBP1-83253-img0001.jpg "Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253]")
Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253]
Immunohistochemistry-Paraffin: Kif2a Antibody [NBP1-83253] - Staining of human testis shows nuclear positivity in cells of seminiferus ducts.Applications for Kif2a Antibody
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kif2a
Alternate Names
HK2, KIF2kinesin-2, kinesin heavy chain member 2, kinesin heavy chain member 2A, Kinesin, heavy chain, 2, Kinesin-2, kinesin-like protein KIF2A, KNS2
Gene Symbol
KIF2A
Additional Kif2a Products
Product Documents for Kif2a Antibody
Product Specific Notices for Kif2a Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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