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KIF3A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05030

Novus Biologicals, part of Bio-Techne
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NBP3-05030-100ul
NBP3-05030-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3). RKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIF3A Antibody - BSA Free

Western Blot: KIF3A AntibodyBSA Free [NBP3-05030]

Western Blot: KIF3A AntibodyBSA Free [NBP3-05030]

Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: KIF3A Antibody - BSA Free [NBP3-05030]

Immunocytochemistry/ Immunofluorescence: KIF3A Antibody - BSA Free [NBP3-05030]

Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation: KIF3A Antibody - BSA Free [NBP3-05030]

Immunoprecipitation: KIF3A Antibody - BSA Free [NBP3-05030]

Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.

Applications for KIF3A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KIF3A

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro

Alternate Names

KIF3, kinesin family member 3A, kinesin family protein 3A, kinesin-like protein KIF3A, Microtubule plus end-directed kinesin motor 3A

Gene Symbol

KIF3A

Additional KIF3A Products

Product Documents for KIF3A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KIF3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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