Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KIFC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 625 and 673 of human Kinesin Family Member C1 (Mitotic Kinesin-Like Protein 2) using the numbering given in entry NP_002254.1 (GeneID 3833).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30804089).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIFC1 Antibody

Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844]

Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844]

Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: KIFC1 Antibody [NB100-40844]

Immunoprecipitation: KIFC1 Antibody [NB100-40844]

Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and NB100-40843.

Applications for KIFC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. WB reactivity reported in (PMID: 28430595). Western Blot Not recommended. Chip, WB reactivity reported in (PMID: 24648347). Use in ICC/IF was reported in scientific literature (PMID: 30804089).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIFC1

KIFC1 is a C-terminal kinesin motor associated with the nuclear membrane and acrosome in round and elongating spermatids. This location in developing spermatids is consistent with possible roles in acrosome elongation and manchette motility or both. It is a minus-end-directed motor enzyme.

Alternate Names

HSETMGC1202, kinesin family member C1, kinesin-like 2, Kinesin-like protein 2, kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2MGC149736, MGC149737

Entrez Gene IDs

3833 (Human)

Gene Symbol

KIFC1

UniProt

Q9BW19 (Human)

Additional KIFC1 Products

Product Documents for KIFC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KIFC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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