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Kinesin 5B Antibody (S04-6G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14868

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-14868-100ul
NBP3-14868-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-6G8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide of human Kinesin 5B (Uniprot#: P33176)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kinesin 5B Antibody (S04-6G8)

Western Blot: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Western Blot: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Western Blot: Kinesin 5B Antibody (S04-6G8) [NBP3-14868] - Western blot detection of Kinesin 5B in Hela, MCF7, U251MG, K562 cell lysates using Kinesin 5B Rabbit mAb (1:500 diluted). Predicted band size: 110kDa. Observed band size: 110kDa.
Immunocytochemistry/ Immunofluorescence: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Immunocytochemistry/ Immunofluorescence: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Immunocytochemistry/Immunofluorescence: Kinesin 5B Antibody (S04-6G8) [NBP3-14868] - Immunofluorescence of Kinesin 5B (green) in Hela using Kinesin 5B Rabbit mAb at dilution 1/5, and DAPI (blue)
Immunohistochemistry-Paraffin: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Immunohistochemistry-Paraffin: Kinesin 5B Antibody (S04-6G8) [NBP3-14868]

Immunohistochemistry-Paraffin: Kinesin 5B Antibody (S04-6G8) [NBP3-14868] - Immunohistochemistry of Kinesin 5B in paraffin-embedded Human breast cancer tissue using Kinesin 5B Rabbit mAb at dilution 1/50

Applications for Kinesin 5B Antibody (S04-6G8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/100

Immunohistochemistry

1/20-1/100

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kinesin 5B

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Alternate Names

Conventional kinesin heavy chain, kinesin family member 5B, kinesin-1 heavy chain, KNS1kinesin 1 (110-120kD), KNSKINH, Ubiquitous kinesin heavy chain, UKHCkinesin heavy chain

Gene Symbol

KIF5B

Additional Kinesin 5B Products

Product Documents for Kinesin 5B Antibody (S04-6G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kinesin 5B Antibody (S04-6G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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