Kir2.2 Antibody (S124B-38)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12901
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S124B-38
Concentration
1 mg/ml
Product Summary for Kir2.2 Antibody (S124B-38)
Immunogen
Fusion protein amino acids 362-427 of rat Kir2.2
Localization
Membrane
Specificity
Detects approx 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Kir2.2 Antibody (S124B-38)
![Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901]](https://resources.bio-techne.com/images/products/Kir2-2-Antibody-S124B-38-Western-Blot-NBP2-12901-img0006.jpg "Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901]")
Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901]
Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901] - analysis of Human Cell lysates showing detection of Kir2.2 Potassium Channel protein using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]](https://resources.bio-techne.com/images/products/Kir2-2-Antibody-S124B-38-Immunohistochemistry-NBP2-12901-img0008.jpg "Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]")
Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]
Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]](https://resources.bio-techne.com/images/products/Kir2-2-Antibody-S124B-38-Immunohistochemistry-NBP2-12901-img0007.jpg "Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]")
Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901]
Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.Applications for Kir2.2 Antibody (S124B-38)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of Kir2.2 Antibody was sufficient for detection of Kir2.2 in 30 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kir2.2
Long Name
ATP-sensitive inward rectifier potassium channel 12
Alternate Names
IRK-2, IRK2, KCNJ12, KCNJN1
Gene Symbol
KCNJ12
Additional Kir2.2 Products
Product Documents for Kir2.2 Antibody (S124B-38)
Product Specific Notices for Kir2.2 Antibody (S124B-38)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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