KIR2DL1/CD158a Antibody (MM0438-11G25) [PE/Atto594]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11758PEATT594

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0438-11G25

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KIR2DL1/CD158a Antibodies »

Product Summary for KIR2DL1/CD158a Antibody (MM0438-11G25) [PE/Atto594]

Immunogen

Human recombinant KIR2DL1/CD158a

Specificity

This antibody detects KIR2DL2, KIR2DL3, KIR2DS2, and KIR2DS4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Applications for KIR2DL1/CD158a Antibody (MM0438-11G25) [PE/Atto594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: KIR2DL1/CD158a

KIRDL2, also known as CD158a, belongs to the killer-cell immunoglobulin-like receptor (KIR) gene family expressed by natural killer (NK) cells and functions in inhibitory signaling (1,2). The KIR genes consisting of 15 gene loci and two pseudogenes are located on chromosome 19 and segregated into halptype A or B depending on gene composition (1). Human KIR2DL1 belongs to haplotype A and is synthesized as a protein of 348 amino acids (aa) in length with a theoretical molecular weight (MW) of 38.5 kDa (3). The KIR2DL1 protein consists of two extracellular immunoglobulin (Ig)-like C2 domains, a transmembrane domain, and a long cytoplasmic tail containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) (1,3). KIR2DL1 binds human leukocyte antigen (HLA) alleles with the C2 supratype, resulting in an inhibitory immune response and suppression of NK cell activity to target cells (1,2). KIR2DL1 has been shown to be a potential clinical biomarker for diseases related to suppressed NK cell function (4). An increase in inhibitory receptors such as KIR2DL1/CD158a is observed in certain pathologies like endometriosis and multiple myeloma (4,5). Therapeutic inhibition of KIR2DL1 may be a potential treatment option for endometriosis patients (5). Additionally, blocking of KIR2DL1 combined with rituximab, and anti-CD20 monoclonal antibody, has shown increased NK cell cytotoxicity than just the antibody itself (5).

References

1. Debska-Zielkowska J, Moszkowska G, Zielinski M, et al. KIR Receptors as Key Regulators of NK Cells Activity in Health and Disease. Cells. 2021;10(7):1777. Published 2021 Jul 14. https://doi.org/10.3390/cells10071777

2. Goodson-Gregg FJ, Krepel SA, Anderson SK. Tuning of human NK cells by endogenous HLA-C expression. Immunogenetics. 2020;72(4):205-215. https://doi.org/10.1007/s00251-020-01161-x

3. Uniprotkb (P43626)

4. Konjevic G, Jurisic V, Jovic V, et al. Investigation of NK cell function and their modulation in different malignancies. Immunol Res. 2012;52(1-2):139-156. https://doi.org/10.1007/s12026-012-8285-7

5. Hoogstad-van Evert J, Paap R, Nap A, van der Molen R. The Promises of Natural Killer Cell Therapy in Endometriosis. Int J Mol Sci. 2022;23(10):5539. Published 2022 May 16. https://doi.org/10.3390/ijms23105539

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 1

Alternate Names

CD158a

Gene Symbol

KIR2DL1

Additional KIR2DL1/CD158a Products

Product Documents for KIR2DL1/CD158a Antibody (MM0438-11G25) [PE/Atto594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KIR2DL1/CD158a Antibody (MM0438-11G25) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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