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KLF11 Antibody (10D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00008462-M03

Novus Biologicals, part of Bio-Techne
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H00008462-M03

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 10D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

KLF11 (NP_003588, 404 a.a. ~ 512 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TYFKSSHLKAHLRTHTGEKPFNCSWDGCDKKFARSDELSRHRRTHTGEKKFVCPVCDRRFMRSDHLTKHARRHMTTKKIPGWQAEVGKLNRIASAESPGSPLVSMPASA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

KLF11 - Kruppel-like factor 11 (10D8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KLF11 Antibody (10D8)

Western Blot: KLF11 Antibody (10D8) [H00008462-M03]

Western Blot: KLF11 Antibody (10D8) [H00008462-M03]

Western Blot: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of KLF11 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: KLF11 Antibody (10D8) [H00008462-M03]

Immunocytochemistry/ Immunofluorescence: KLF11 Antibody (10D8) [H00008462-M03]

Immunocytochemistry/Immunofluorescence: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on NIH/3T3 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: KLF11 Antibody (10D8) [H00008462-M03]

Immunohistochemistry-Paraffin: KLF11 Antibody (10D8) [H00008462-M03]

Immunohistochemistry-Paraffin: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml

Applications for KLF11 Antibody (10D8)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, ELISA, ChIP and IHC-P. Use in Immunoprecipitation reported in scientific literature (PMID: 23408111).

Reviewed Applications

Read 1 review rated 4 using H00008462-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KLF11

Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis

Alternate Names

FKLFFKLF1, Krueppel-like factor 11, Kruppel-like factor 11, MODY7TGFB-inducible early growth response protein 2, TIEG-2, TIEG2TGFB inducible early growth response 2, Tieg3, Transforming growth factor-beta-inducible early growth response protein 2

Entrez Gene IDs

8462 (Human)

Gene Symbol

KLF11

Additional KLF11 Products

Product Documents for KLF11 Antibody (10D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF11 Antibody (10D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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