KLF15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24635

Novus Biologicals, part of Bio-Techne
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NBP2-24635
NBP2-24635SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human, Mouse, Rat, Bovine

Predicted:

Porcine (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KLF15 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human KLF15 protein (between amino acids 100-200) [UniProt Q9UIH9]

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29551501). Use in Mouse reported in scientific literature (PMID:32437957).

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF15 Antibody

Western Blot: KLF15 Antibody [NBP2-24635]

Western Blot: KLF15 Antibody [NBP2-24635]

Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: KLF15 Antibody [NBP2-24635]

Immunocytochemistry/ Immunofluorescence: KLF15 Antibody [NBP2-24635]

Immunocytochemistry/Immunofluorescence: KLF15 Antibody [NBP2-24635] - KLF15 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635]

Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635]

Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635] - Tissue section of human liver using KLF15 antibody at 1:100 dilution. The antibody generated strong cytoplasmic staining with some nuclear signal in the hepatocytes, while in Kupffer cells, the signal was mainly nuclear and was most intense among all cell types.

Applications for KLF15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

1.0-2.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF15

The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

Alternate Names

DKFZp779M1320, kidney-enriched Kruppel-like factor, KKLFKidney-enriched krueppel-like factor, Krueppel-like factor 15, Kruppel-like factor 15

Entrez Gene IDs

28999 (Human)

Gene Symbol

KLF15

UniProt

Q9UIH9 (Human)

Additional KLF15 Products

Product Documents for KLF15 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KLF15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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