KLF2 Antibody (OTI3A10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71597

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all KLF2 Antibodies »

Product Summary for KLF2 Antibody (OTI3A10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human KLF2 (NP_057354) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF2 Antibody (OTI3A10) - Azide and BSA Free

Western Blot: KLF2 Antibody (OTI3A10)Azide and BSA Free [NBP2-71597]

Western Blot: KLF2 Antibody (OTI3A10)Azide and BSA Free [NBP2-71597]

Western Blot: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF2.
Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597]

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597]

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)
Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597]

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597]

Immunohistochemistry: KLF2 Antibody (OTI3A10) - Azide and BSA Free [NBP2-71597] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for KLF2 Antibody (OTI3A10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF2

KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Long Name

Kruppel-Like Factor 2

Alternate Names

LKLF

Gene Symbol

KLF2

Additional KLF2 Products

Product Documents for KLF2 Antibody (OTI3A10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KLF2 Antibody (OTI3A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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