KLF8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57740
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQ
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31827393).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KLF8 Antibody
Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]
Immunocytochemistry/Immunofluorescence: KLF8 Antibody [NBP2-57740] - Staining of human cell line RT4 shows localization to nucleoplasm & aggresome.Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]
Immunocytochemistry/Immunofluorescence: KLF8 Antibody [NBP2-57740] - DANCR knockdown blocked the tumor formation in vivo involving KLF8 activation. CAL-27 or TCa-8113 cells transfected with shRNA against DANCR were inoculated subcutaneously into the nude mice. Xenografts were measured every 4 days with a caliper. Immunofluorescence staining was performed to investigate KLF8 immunoreactive materials in tumor tissues after 25 days. ***p < 0.001, versus to sh-NC Image collected and cropped by CiteAb from the following publication (https://cancerci.biomedcentral.com/articles/10.1186/s12935-019-1016-6), licensed under a CC-BY license.Western Blot: KLF8 Antibody [NBP2-57740] -
Western Blot: KLF8 Antibody [NBP2-57740] - MiR-135a-5p overexpression suppressed tumor cell progression & KLF8 expression in vitro. a Relative expression of miR-135a-5p in different TSCC cell lines was examined using qRT-PCR. b Relative expression of miR-135a-5p was measured in CAL-27 & TCa-8113 cells transfected with miR-135a-5p mimics by qRT-PCR. c The viability of CAL-27 & TCa-8113 cells was measured using MTT assay. d, e The migration & invasion of CAL-27 & TCa-8113 cells was examined using wound healing assay & transwell assay, respectively. f Relative expression of KLF8 mRNA was detected in CAL-27 & TCa-8113 cells using qRT-PCR. g Relative expression of KLF8 protein was measured using western blot in CAL-27 & TCa-8113 cells. *p < 0.05, **p < 0.01, ***p < 0.001, versus to NC mimics Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for KLF8 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Paraffin
Use in IHC-P reported in scientific literature (PMID:31827393).
Western Blot
Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY licence.
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF8
Alternate Names
Basic krueppel-like factor 3, basic kruppel-like factor 3, BKLF3DKFZp686O08126, DXS741, Krueppel-like factor 8, Kruppel-like factor 8, Zinc finger protein 741, ZNF741MGC138314
Gene Symbol
KLF8
Additional KLF8 Products
Product Documents for KLF8 Antibody
Product Specific Notices for KLF8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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