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KLF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57740

Novus Biologicals, part of Bio-Techne
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NBP2-57740
NBP2-57740-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQ

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31827393).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF8 Antibody

Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]

Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]

Immunocytochemistry/Immunofluorescence: KLF8 Antibody [NBP2-57740] - Staining of human cell line RT4 shows localization to nucleoplasm & aggresome.
Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]

Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]

Immunocytochemistry/Immunofluorescence: KLF8 Antibody [NBP2-57740] - DANCR knockdown blocked the tumor formation in vivo involving KLF8 activation. CAL-27 or TCa-8113 cells transfected with shRNA against DANCR were inoculated subcutaneously into the nude mice. Xenografts were measured every 4 days with a caliper. Immunofluorescence staining was performed to investigate KLF8 immunoreactive materials in tumor tissues after 25 days. ***p < 0.001, versus to sh-NC Image collected and cropped by CiteAb from the following publication (https://cancerci.biomedcentral.com/articles/10.1186/s12935-019-1016-6), licensed under a CC-BY license.
KLF8 Antibody

Western Blot: KLF8 Antibody [NBP2-57740] -

Western Blot: KLF8 Antibody [NBP2-57740] - MiR-135a-5p overexpression suppressed tumor cell progression & KLF8 expression in vitro. a Relative expression of miR-135a-5p in different TSCC cell lines was examined using qRT-PCR. b Relative expression of miR-135a-5p was measured in CAL-27 & TCa-8113 cells transfected with miR-135a-5p mimics by qRT-PCR. c The viability of CAL-27 & TCa-8113 cells was measured using MTT assay. d, e The migration & invasion of CAL-27 & TCa-8113 cells was examined using wound healing assay & transwell assay, respectively. f Relative expression of KLF8 mRNA was detected in CAL-27 & TCa-8113 cells using qRT-PCR. g Relative expression of KLF8 protein was measured using western blot in CAL-27 & TCa-8113 cells. *p < 0.05, **p  < 0.01, ***p < 0.001, versus to NC mimics Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KLF8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Paraffin

Use in IHC-P reported in scientific literature (PMID:31827393).

Western Blot

Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY licence.
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF8

The KLF8 gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in t

Alternate Names

Basic krueppel-like factor 3, basic kruppel-like factor 3, BKLF3DKFZp686O08126, DXS741, Krueppel-like factor 8, Kruppel-like factor 8, Zinc finger protein 741, ZNF741MGC138314

Gene Symbol

KLF8

Additional KLF8 Products

Product Documents for KLF8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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