KLH Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97336
Key Product Details
Species Reactivity
All Species
Applications
ELISA, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KLH (Keyhole Limpet Hemocyanin)
Localization
Secreted protein; extracellular space
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Substrate dependent
Conjugation Chemistry: Glutaraldehye coupling
This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and KLH.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Substrate dependent
Conjugation Chemistry: Glutaraldehye coupling
This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and KLH.
Scientific Data Images for KLH Antibody [Alkaline Phosphatase]
Western Blot: KLH Antibody [Alkaline Phosphatase] [NBP1-97336] - Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 ug. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.
Applications for KLH Antibody [Alkaline Phosphatase]
Application
Recommended Usage
ELISA
1:15000-1:60000
Western Blot
1:100-1:2000
Application Notes
This product has been assayed against 1.0 ug of KLH in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:13,500 is suggested for this product.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: KLH
Alternate Names
keyhole limpet hemocyanin (KLH)
Additional KLH Products
Product Documents for KLH Antibody [Alkaline Phosphatase]
Product Specific Notices for KLH Antibody [Alkaline Phosphatase]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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