KLHL2 Antibody (OTI1G7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71695

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all KLHL2 Antibodies »

Product Summary for KLHL2 Antibody (OTI1G7) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLHL2 Antibody (OTI1G7) - Azide and BSA Free

Western Blot: KLHL2 Antibody (OTI1G7)Azide and BSA Free [NBP2-71695]

Western Blot: KLHL2 Antibody (OTI1G7)Azide and BSA Free [NBP2-71695]

Western Blot: KLHL2 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71695]

Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71695]

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.
Western Blot: KLHL2 Antibody (OTI1G7)Azide and BSA Free [NBP2-71695]

Western Blot: KLHL2 Antibody (OTI1G7)Azide and BSA Free [NBP2-71695]

Western Blot: KLHL2 Antibody (OTI1G7) - Azide and BSA Free [NBP2-71695] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.
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Applications for KLHL2 Antibody (OTI1G7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLHL2

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Alternate Names

ABP-KELCH, Actin-binding protein Mayven, kelch, kelch (Drosophila)-like 2 (Mayven), kelch-like 2, Mayven (Drosophila), kelch-like protein 2, MAV, MAYVEN

Gene Symbol

KLHL2

Additional KLHL2 Products

Product Documents for KLHL2 Antibody (OTI1G7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KLHL2 Antibody (OTI1G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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