KLHL25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83974

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-83974
NBP1-83974-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KLHL25 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HFETVRQSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLHL25 Antibody

Western Blot: KLHL25 Antibody [NBP1-83974]

Western Blot: KLHL25 Antibody [NBP1-83974]

Western Blot: KLHL25 Antibody [NBP1-83974] - Analysis in control (vector only transfected HEK293T lysate) and KLHL25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody [NBP1-83974]

Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody [NBP1-83974]

Immunocytochemistry/Immunofluorescence: KLHL25 Antibody [NBP1-83974] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974]

Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974]

Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for KLHL25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLHL25

ENC2( AAH28100, 1 a.a. - 590 a.a.) full-length recombinant protein with GST.

Alternate Names

ectodermal-neural cortex 2, ENC-2BTB/POZ KELCH domain protein, ENC2ectoderm-neural cortex protein 2, FLJ12587, FLJ45015, kelch-like 25 (Drosophila), Kelch-like protein 25

Gene Symbol

KLHL25

Additional KLHL25 Products

Product Documents for KLHL25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KLHL25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.