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Klotho Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76511

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the center of human Klotho. The immunogen is located within amino acids 410-460 of Klotho. Amino Acid Sequence: SGTTKRRDDAKYMYYLK

Specificity

Three isoforms of Klotho are known to exist. Human Klotho has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse Klotho has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat Klotho has one isoform (1014aa, 117kD). NBP1-76511 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Klotho Antibody - BSA Free

Western Blot: Klotho AntibodyBSA Free [NBP1-76511]

Western Blot: Klotho AntibodyBSA Free [NBP1-76511]

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Klotho Antibody - BSA Free

Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] -

Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511]

Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511]

Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.

Applications for Klotho Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-4 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Klotho

KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

Alternate Names

KL

Gene Symbol

KL

Additional Klotho Products

Product Documents for Klotho Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Klotho Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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