Klotho Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76511
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of human Klotho. The immunogen is located within amino acids 410-460 of Klotho. Amino Acid Sequence: SGTTKRRDDAKYMYYLK
Specificity
Three isoforms of Klotho are known to exist. Human Klotho has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse Klotho has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat Klotho has one isoform (1014aa, 117kD). NBP1-76511 can detect human, mouse and rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Klotho Antibody - BSA Free
Western Blot: Klotho AntibodyBSA Free [NBP1-76511]
Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] -
Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart TissueImmunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511]
Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.Applications for Klotho Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2.5 ug/mL
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1-4 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Klotho
Additional Klotho Products
Product Documents for Klotho Antibody - BSA Free
Product Specific Notices for Klotho Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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