KMT1A/SUV39H1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21367

Novus Biologicals, part of Bio-Techne
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NBP1-21367-20ug
NBP1-21367
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all KMT1A/SUV39H1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human KMT1A/SUV39H1 protein (between residues 1-100) [UniProt O43463]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28425504).

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT1A/SUV39H1 Antibody

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367]

Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367]

Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.
Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.
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Applications for KMT1A/SUV39H1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:1500

Immunoprecipitation

5- 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
IHC-P, WB reactivitiy reported in scientific literature (PMID: 23943221), and verified customer reviews. Use in ICC/IF reported in scientific literature (PMID:28425504).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3.8 using NBP1-21367 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KMT1A/SUV39H1

SUV39H1 (suppressor of variegation 3-9 homolog 1) is a histone methyltransferase that contains a chromodomain and a C-terminal SET domain. It specifically trimethylates histone H3 at lys 9. Interaction of SUV39H1 with RB1 results in recruitment to histone H3. Trimethylation of H3 stimulates the binding of heterochromatin proteins ((CBX1, CBX3 and/or CBX5) which leads to epigenetic transcriptional repression in heterochromatin regions. Recent research has also shown that this protein interacts with oncogenes involved in AML. Treatment of AML cells with chaetocin caused induction of apotosis via direct and indirect modulation of SUV39H1 activity (PMID:21979880). This protein plays a critical role in heterochromatin organization, chromosome segregation, and mitotic progression.

Long Name

Histone-lysine N-methyltransferase SUV39H1

Alternate Names

EC 2.1.1.355, H3-K9-HMTase 1, KMT1A, SUV39H, SUV39H1

Entrez Gene IDs

6839 (Human)

Gene Symbol

SUV39H1

UniProt

O43463 (Human)

Additional KMT1A/SUV39H1 Products

Product Documents for KMT1A/SUV39H1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KMT1A/SUV39H1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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