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KMT2A/MLL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-256

Novus Biologicals, part of Bio-Techne
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NB600-256

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 2725 and 2775 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT2A/MLL Antibody

Western Blot: KMT2A/MLL Antibody [NB600-256]

Western Blot: KMT2A/MLL Antibody [NB600-256]

Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Flow Cytometry: KMT2A/MLL Antibody [NB600-256]

Flow Cytometry: KMT2A/MLL Antibody [NB600-256]

Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).
Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).

Applications for KMT2A/MLL Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-5 ug/ml

Immunoprecipitation

2-5 ug/mg of lysate

Simple Western

1:10

Western Blot

1:2000-1:10000
Application Notes
KMT2A/MLL antibody validated for FLOW from a verified customer review. Simple Western reported by an internal validation: tested in MCF-7, T-47D cell lines; antibody dilution of 1:10; separated by size

Reviewed Applications

Read 2 reviews rated 4 using NB600-256 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KMT2A/MLL

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Alternate Names

ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX

Entrez Gene IDs

4297 (Human)

Gene Symbol

KMT2A

UniProt

Additional KMT2A/MLL Products

Product Documents for KMT2A/MLL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KMT2A/MLL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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