Skip to main content

KSHV ORF57 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95260

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-95260
NBP2-95260-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Virus

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This affinity purified KSHV ORF57 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein. (Uniprot: Q2HR75)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with KSHV ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown

Localization

Tumor progenitor endothelial cells

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Scientific Data Images for KSHV ORF57 Antibody

Western Blot: KSHV ORF57 Antibody [NBP2-95260]

Western Blot: KSHV ORF57 Antibody [NBP2-95260]

Western Blot: KSHV ORF57 Antibody [NBP2-95260] - Western blot using KSHV ORF57 Antibody to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7,500 (left) and 1:1,000 (right).

Applications for KSHV ORF57 Antibody

Application
Recommended Usage

ELISA

1:100000-170000

Western Blot

1:5000-1:8000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: KSHV ORF57

Alternate Names

herpesvirus core gene UL54 family, ORF57

Gene Symbol

ORF57

UniProt

Additional KSHV ORF57 Products

Product Documents for KSHV ORF57 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KSHV ORF57 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...