KSHV ORF57 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95260
Key Product Details
Validated by
Biological Validation
Species Reactivity
Virus
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified KSHV ORF57 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein. (Uniprot: Q2HR75)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with KSHV ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown
Localization
Tumor progenitor endothelial cells
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Scientific Data Images for KSHV ORF57 Antibody
Western Blot: KSHV ORF57 Antibody [NBP2-95260]
Western Blot: KSHV ORF57 Antibody [NBP2-95260] - Western blot using KSHV ORF57 Antibody to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7,500 (left) and 1:1,000 (right).Applications for KSHV ORF57 Antibody
Application
Recommended Usage
ELISA
1:100000-170000
Western Blot
1:5000-1:8000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: KSHV ORF57
Additional KSHV ORF57 Products
Product Documents for KSHV ORF57 Antibody
Product Specific Notices for KSHV ORF57 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...