Ku70/XRCC6 Antibody (8L6B6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15358
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8L6B6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-609 of human Ku70/XRCC6 (P12956). PEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ku70/XRCC6 Antibody (8L6B6)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]](https://resources.bio-techne.com/images/products/Ku70-XRCC6-Antibody-8L6B6-Immunohistochemistry-Paraffin-NBP3-15358-img0001.jpg "Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]")
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358]
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ku70/XRCC6 Rabbit mAb (NBP3-15358) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -
Western Blot: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] - Analysis of lysates from A-549 cells using Ku70 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -
Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (8L6B6) [NBP3-15358] -Confocal imaging of U-2 OS cells using Ku70 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Applications for Ku70/XRCC6 Antibody (8L6B6)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:200 - 1:800
Immunohistochemistry-Paraffin
1:200 - 1:800
Western Blot
1:6000 - 1:36000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ku70/XRCC6
Long Name
Lupus Ku Autoantigen Protein 70
Alternate Names
CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6
Gene Symbol
XRCC6
Additional Ku70/XRCC6 Products
Product Documents for Ku70/XRCC6 Antibody (8L6B6)
Product Specific Notices for Ku70/XRCC6 Antibody (8L6B6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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