Ku80/XRCC5 Antibody (3D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00007520-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Quantification , Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ku80/XRCC5 Antibodies »

Product Specifications

Immunogen

XRCC5 (AAH19027.1, 1 a.a. ~ 732 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADSLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGIALITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Localization

Nuclear

Specificity

XRCC5 - X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ku80/XRCC5 Antibody (3D8)

Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - XRCC5 monoclonal antibody (M02), clone 3D8. Analysis of XRCC5 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
View 2 more images

Applications for Ku80/XRCC5 Antibody (3D8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Quantification

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ku80/XRCC5

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Long Name

Lupus Ku Autoantigen Protein 86

Alternate Names

CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5

Entrez Gene IDs

7520 (Human)

Gene Symbol

XRCC5

OMIM

194364 (Human)

UniProt

AAH19027 (Human)

Additional Ku80/XRCC5 Products

Product Documents for Ku80/XRCC5 Antibody (3D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ku80/XRCC5 Antibody (3D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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