Ku80/XRCC5 Antibody (7N7G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15448

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15448-100ul
NBP3-15448-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7N7G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ku80/XRCC5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80/XRCC5 (P13010). MKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ku80/XRCC5 Antibody (7N7G3)

Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Western blot analysis of extracts of various cell lines, using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunofluorescence analysis of U-2 OS cells using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunohistochemistry of paraffin-embedded human esophageal using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Ku80/XRCC5 Antibody (7N7G3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ku80/XRCC5

Ku80, also known as XRCC5, plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis.

As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.

Long Name

Lupus Ku Autoantigen Protein 86

Alternate Names

CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5

Gene Symbol

XRCC5

Additional Ku80/XRCC5 Products

Product Documents for Ku80/XRCC5 Antibody (7N7G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ku80/XRCC5 Antibody (7N7G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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