Ku80/XRCC5 Antibody (XRCC5/7316)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20328
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG2b Kappa Clone # XRCC5/7316
Concentration
0.2 mg/ml
Product Summary for Ku80/XRCC5 Antibody (XRCC5/7316)
Immunogen
Recombinant fragment (around aa300-500) of human Ku80/XRCC5 (exact sequence is proprietary)
Localization
Nucleus. Nucleoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG2b Kappa
Description
Positive Controls: HeLa, MOLT-4, MCF-7 or HT29 cells. Human thyroid or lymph node.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Ku80/XRCC5 Antibody (XRCC5/7316)
Western blot analysis of MOLT-4 cell lysates using Ku80/XRCC5 antibody (XRCC5/7316).
Ku80/XRCC5 Antibody (XRCC5/7316)
Western blot analysis of MOLT-4 cell lysates using Ku80/XRCC5 antibody (XRCC5/7316).
Ku80/XRCC5 Antibody (XRCC5/7316)
Formalin-fixed, paraffin-embedded human lymph node stained with Ku80/XRCC5 antibody (XRCC5/7316). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Ku80/XRCC5 Antibody (XRCC5/7316)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Ku80/XRCC5
As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.
Long Name
Lupus Ku Autoantigen Protein 86
Alternate Names
CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5
Gene Symbol
XRCC5
Additional Ku80/XRCC5 Products
Product Documents for Ku80/XRCC5 Antibody (XRCC5/7316)
Product Specific Notices for Ku80/XRCC5 Antibody (XRCC5/7316)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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