Ku80/XRCC5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-508
![Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0005.jpg "Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Hamster
Cited:
Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein.
Specificity
Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ku80/XRCC5 Antibody - BSA Free
Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]
Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.![Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0004.jpg "Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]")
Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]
Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.![Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-508]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Immunoprecipitation-NB100-508-img0003.jpg "Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-508]")
Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-508]
Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.Applications for Ku80/XRCC5 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) This antibody has only been tested in Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB100-508 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ku80/XRCC5
Long Name
Lupus Ku Autoantigen Protein 86
Alternate Names
CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5
Gene Symbol
XRCC5
Additional Ku80/XRCC5 Products
Product Documents for Ku80/XRCC5 Antibody - BSA Free
Product Specific Notices for Ku80/XRCC5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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