Ku80/XRCC5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87829
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTE
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ku80/XRCC5 Antibody
![Western Blot: Ku80/XRCC5 Antibody [NBP1-87829]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Western-Blot-NBP1-87829-img0005.jpg "Western Blot: Ku80/XRCC5 Antibody [NBP1-87829]")
Western Blot: Ku80/XRCC5 Antibody [NBP1-87829]
Western Blot: Ku80/XRCC5 Antibody [NBP1-87829] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-306![Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87829-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829]")
Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829]
Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-87829]](https://resources.bio-techne.com/images/products/Ku80-XRCC5-Antibody-Immunohistochemistry-Paraffin-NBP1-87829-img0002.jpg "Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-87829]")
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-87829]
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-87829] - Staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.Applications for Ku80/XRCC5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ku80/XRCC5
As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.
Long Name
Lupus Ku Autoantigen Protein 86
Alternate Names
CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5
Gene Symbol
XRCC5
Additional Ku80/XRCC5 Products
Product Documents for Ku80/XRCC5 Antibody
Product Specific Notices for Ku80/XRCC5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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