Kv7.1 Antibody (S37A/10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12897
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Hamster
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S37A/10
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 2-101 of human KCNQ1
Localization
Cell Membrane, Cytoplasmic Vesicle Membrane
Specificity
Detects approx 75 kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Kv7.1 Antibody (S37A/10)
Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897]
Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Western Blot analysis of Human Cell lysates showing detection of Kv7.1 protein using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/ Immunofluorescence: Kv7.1 Antibody (S37A/10) [NBP2-12897]
Immunocytochemistry/Immunofluorescence: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.1 Antibody (D) Composite.Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897]
Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunohistochemistry analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.Applications for Kv7.1 Antibody (S37A/10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of KCNQ1 Antibody was sufficient for detection of KCNQ1 in 10 ug of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kv7.1
Long Name
Potassium voltage-gated channel subfamily KQT member 1
Alternate Names
KCNA8, KCNA9, KCNQ1, KQT-like 1, KVLQT1
Gene Symbol
KCNQ1
Additional Kv7.1 Products
Product Documents for Kv7.1 Antibody (S37A/10)
Product Specific Notices for Kv7.1 Antibody (S37A/10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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