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Lactate Dehydrogenase C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68159

Novus Biologicals, part of Bio-Techne
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NB100-68159

Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KLGTDSDKEHWKNIH corresponding to internal region according to NP_002292.1, NP_059144.1.

Epitope

KLGTDSDKEHWKNIH

Specificity

Both variants represent identical product (NP_002292.1 and NP_059144.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lactate Dehydrogenase C Antibody

Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159]

Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159]

Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159] - Lactate Dehydrogenase C Antibody [NB100-68159] (0.03ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159] - (1ug/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.

Applications for Lactate Dehydrogenase C Antibody

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:16000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 30-35 kDa band observed in mouse testis lysates (calculated MW of 36.6 kDa band according to human NP_002292.1 and 35.9 kDa band according to mouse NP_038608.1). IHC: Strong signal in seminiferous tubules of mouse testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase C

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Alternate Names

Cancer/testis antigen 32, CT32EC 1.1.1.27, EC 1.1.1, lactate dehydrogenase C, lactate dehydrogenase C4, LDH testis subunit, LDH3MGC111073, LDH-C, LDHX, LDH-X, L-lactate dehydrogenase C chain

Entrez Gene IDs

3948 (Human); 16833 (Mouse)

Gene Symbol

LDHC

Additional Lactate Dehydrogenase C Products

Product Documents for Lactate Dehydrogenase C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lactate Dehydrogenase C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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