LAIR1 Antibody (PSH04-50)

Western Blot: LAIR1 Antibody (PSH04-50) [NBP3-32517]

Western Blot: LAIR1 Antibody (PSH04-50) [NBP3-32517] - Western blot analysis of LAIR1 on different lysates with Mouse anti-LAIR1 antibody (NBP3-32517) at 1/2,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: HeLa cell lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 32 kDa
Observed band size: 40-45 kDa

Exposure time: 30 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32517) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: LAIR1 Antibody (PSH04-50) [NBP3-32517]

Immunohistochemistry: LAIR1 Antibody (PSH04-50) [NBP3-32517] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-LAIR1 antibody (NBP3-32517) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.