Lambda Light Chain Antibody (4C2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45071

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C2

Format

BSA Free

Concentration

1 mg/ml

View all Lambda Light Chain Antibodies »

Product Specifications

Immunogen

The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.

Specificity

The mouse monoclonal antibody H4B4 recognizes CD107b / LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lambda Light Chain Antibody (4C2) - BSA Free

Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.
Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.
Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) - BSA Free [NBP1-45071]

Flow Cytometry: Lambda Light Chain Antibody (4C2) [NBP1-45071] - Surface staining of human peripheral blood with anti-human lambda light chain (4C2) FITC.
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Applications for Lambda Light Chain Antibody (4C2) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

2-8 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lambda Light Chain

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody (4C2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lambda Light Chain Antibody (4C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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