Lambda Light Chain Antibody (RM127) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62021B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM127

Concentration

1 mg/ml

View all Lambda Light Chain Antibodies »

Product Summary for Lambda Light Chain Antibody (RM127) [Biotin]

Immunogen

Human IgG

Specificity

This antibody reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lambda Light Chain Antibody (RM127) [Biotin]

Immunohistochemistry: Lambda Light Chain Antibody (RM127) [Biotin] [NBP2-62021B] - Immunohistochemistry of Human Tonsil using Anti-Lambda Light Chain antibody NBP2-62021B.
ELISA: Lambda Light Chain Antibody (RM127) [Biotin] [NBP2-62021B] - ELISA of human immunoglobulins shows NBP2-62021B reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immuno-globulins.NBP2-62021B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: Lambda Light Chain Antibody (RM127) [Biotin] [NBP2-62021B] - A titer ELISA using NBP2-62021B. The plate was coated with different amounts of human IgG3 lambda. A serial dilution of NBP2-62021B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for Lambda Light Chain Antibody (RM127) [Biotin]

Application
Recommended Usage

ELISA

0.05ug/ml - 0.2ug/ml

Flow Cytometry

0.2ug/ml-2ug/ml

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody (RM127) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lambda Light Chain Antibody (RM127) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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