Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34424

Novus Biologicals, part of Bio-Techne
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NBP2-34424-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM559

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Lambda Light Chain Antibodies »

Product Summary for Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

Immunogen

Purified human Lambda Light Chain

Localization

Cell surface, cytoplasmic and secreted

Specificity

This monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34377).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

Western Blot: Lambda Light Chain Antibody (SPM559)Azide and BSA Free [NBP2-34424]

Western Blot: Lambda Light Chain Antibody (SPM559)Azide and BSA Free [NBP2-34424]

Western Blot: Lambda Light Chain Antibody (SPM559) - Azide and BSA Free [NBP2-34424] - Western blot analysis of Lambda Light Chain in human intestine using Lambda Light Chain Ab (SPM559).
Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (SPM559) - Azide and BSA Free [NBP2-34424]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (SPM559) - Azide and BSA Free [NBP2-34424]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (SPM559) - Azide and BSA Free [NBP2-34424] - Formalin-fixed, paraffin-embedded human Tonsil stained with Lambda Light Chain Antibody (SPM559).

Applications for Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lambda Light Chain Antibody (SPM559) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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