Lambda Light Chain Antibody [TRITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64944

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

TRITC (Excitation = 547 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all Lambda Light Chain Antibodies »

Product Summary for Lambda Light Chain Antibody [TRITC]

Immunogen

Mouse paraproteins with lambda 1 and lambda 2 light chains

Reactivity Notes

Human reactivity reported in (PMID: 31350616).

Specificity

LAMBDA LIGHT CHAIN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lambda Light Chain Antibody [TRITC]

Flow Cytometry: Lambda Light Chain Antibody [TRITC] [NB100-64944] - Analysis of peripheral blood lymphocytes by multiple staining.
Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [TRITC] [NB100-64944] - FLISA plate was coated with purified human IgGk, IgMk, IgAk, IgG alpha, IgM alpha IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-TRITC

Applications for Lambda Light Chain Antibody [TRITC]

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Fluorophore-linked immunosorbent assay

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody [TRITC]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lambda Light Chain Antibody [TRITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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