Lamin A + C Antibody (4C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25151

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C4

Concentration

1 mg/ml

View all Lamin A + C Antibodies »

Product Specifications

Immunogen

Full length recombinant human lamin A expressed in and purified from E. coli. [UniProt# P02545]

Reactivity Notes

Monkey and Mouse reactivity reported in scientific literature (PMID:31871052).

Localization

Nucleus. Nucleus envelope.

Marker

Nuclear Envelope Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

74/65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin A + C Antibody (4C4)

Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151]

Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151]

Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

Applications for Lamin A + C Antibody (4C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000-1:2000
Application Notes
This Lamin A + C (4C4) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands can be seen at ~74 kDa (Lamin A) and ~65 kDa (Lamin C).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin A + C

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The Lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some Laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of Lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging.

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Entrez Gene IDs

4000 (Human)

Gene Symbol

LMNA

OMIM

150330 (Human)

UniProt

P02545 (Mouse)

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (4C4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lamin A + C Antibody (4C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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