Lamin B2 Antibody (J9-C4-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32520

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32520
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # J9-C4-R

Concentration

1 mg/ml

View all Lamin B2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Lamin B2 aa 571-620 / 620. (Uniprot: Q03252)

Localization

Nucleus lamina.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin B2 Antibody (J9-C4-R)

Lamin B2 Antibody (J9-C4-R)

Western Blot: Lamin B2 Antibody (J9-C4-R) [NBP3-32520]

Western Blot: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Western blot analysis of Lamin B2 on different lysates with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HCT 116 cell lysate (20 ug/Lane)
Lane 3: HepG2 cell lysate (20 ug/Lane)
Lane 4: COS-1 cell lysate (20 ug/Lane)
Lane 5: Human brain tissue lysate (30 ug/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Lamin B2 Antibody (J9-C4-R)

Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520]

Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Immunofluorescence analysis of paraffin-embedded human brain tissue labeling Lamin B2 with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32520, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Lamin B2 Antibody (J9-C4-R)

Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520]

Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for Lamin B2 Antibody (J9-C4-R)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B2

An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organized into loops, which are attached to the nuclear matrix. This organization is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.

Alternate Names

lamin B2, lamin-B2, LMN2LAMB2, MGC2721

Gene Symbol

LMNB2

Additional Lamin B2 Products

Product Documents for Lamin B2 Antibody (J9-C4-R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lamin B2 Antibody (J9-C4-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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