Lamin B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19325

Novus Biologicals, part of Bio-Techne
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NBP2-19325
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lamin B2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (84%).

Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Marker

Nuclear Envelope Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin B2 Antibody

Western Blot: Lamin B2 Antibody [NBP2-19325]

Western Blot: Lamin B2 Antibody [NBP2-19325]

Western Blot: Lamin B2 Antibody [NBP2-19325] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody [NBP2-19325]

Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody [NBP2-19325]

Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody [NBP2-19325] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lamin B2 antibody (Green) at 1:500 dilution.
Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP2-19325]

Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP2-19325]

Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP2-19325] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.

Applications for Lamin B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lamin B2

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Alternate Names

lamin B2, lamin-B2, LMN2LAMB2, MGC2721

Gene Symbol

LMNB2

Additional Lamin B2 Products

Product Documents for Lamin B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lamin B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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